Mohammad Ayoub

2 Questions 4 Answers 0 Followers

Questions related from Mohammad Ayoub

Why are there sticky mitotic cells in cell culture plates?

Does anybody have any experience with sticky mitotic HeLa cells in the plates? I am growing many 15cm plates for collecting as much mitotic cells I can have for pull-down assay. But it seems that...

13 May 2015 5,775 5 View

Can anyone help me with DpnI-mediated site directed mutagenesis?

I am trying to generate cell lines carrying phosphomimetic substitutions. I have 5 primer pairs, each are ~60 bases.I am using 2.5 micro Molar (uM) forward and the same for rev. primer amd 50ng...

02 April 2015 4,655 9 View