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Does anybody have any experience with sticky mitotic HeLa cells in the plates? I am growing many 15cm plates for collecting as much mitotic cells I can have for pull-down assay. But it seems that...
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I am trying to generate cell lines carrying phosphomimetic substitutions. I have 5 primer pairs, each are ~60 bases.I am using 2.5 micro Molar (uM) forward and the same for rev. primer amd 50ng...
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