Mohamed Nabeel Malash

4 Questions 9 Answers 0 Followers

Questions related from Mohamed Nabeel Malash

Are there any bioinformatics tools for prediction of optimal conditions for enzyme activity?

Are there any bioinformatics tools that can predict the optimal temperature, optimal pH for enzymes activity, based on enzyme sequence or 3D structure?

04 April 2019 2,034 15 View

Pellet of BL21(DE3) washing with 20mM Tris-HCl pH 8?

In pET sytem manual, it is stated that after protein expression and during harvesting cells of BL21 (DE3), you should wash the pellet with 20mM Tris-HCl pH 8. Does anyone know the reason for this?

04 April 2018 5,285 4 View

I wanna verify the identity of pET 28a+. It gives no bands in PCR using T7 pair of primers using different annealing temps. Does sequencing help?

Verification of empty pET28a+ identity before ligation of insert.

12 December 2017 942 3 View

Can a researcher publish a paper twice but in different languages?

legality of double publishing in different languages.

07 July 2017 8,446 4 View