Mohamed Farghaly

5 Questions 6 Answers 0 Followers

Questions related from Mohamed Farghaly

Is it possible to preserve pieces of tissues (not cell suspension) in liquid nitrogen for long term?

To preserve viable tissue for long term, we need to make it in the form of cell suspension then we put in liquid nitrogen, but I need to keep the tissue in the form of pieces not cell suspension,I...

08 August 2015 1,228 9 View

In establishing cell culture, which supplements are essential and can affect the validity of my culture and which ones I can continue if absent ?

FBS,Hydrocortisone, Epidermal Growth Factors(EGF), Insulin, Cholera toxins, and other supplements are used in establishing cell culture. some protocols used all of them, others used some of them,...

12 December 2014 7,886 2 View

Breast tissue enzymatic disaggregation : Trypsin or collagenase??

For breast tissue enzymatic disaggregation what do you prefer Trypsin or collagenase and what are precautions I should take in choosing the media and other reagents for every one of them. Thanks

09 September 2014 7,088 3 View

What is the best protocol for primary culture of normal human breast cancer cells?

As I was looking for a protocol for primary culture of human breast cancer cells, I found widely variable protocols concerning reagents and steps so I wanted to know the stable and common protocol...

09 September 2014 7,686 5 View

How can I measure the effect of aromatase inhibitors in vitro?

Can any one help me in the methods by which I can detect the effect of aromatase inhibitors in treating breast cancer in vitro (Cell culture and induced cancers in mice). Thanks

09 September 2014 3,315 2 View