Mohamed Abu-farha

11 Questions 16 Answers 0 Followers

Questions related from Mohamed Abu-farha

How do you confirm the knockout by CRISPER/Cas9 system?

We are interested in generating knockout in cell lines using the CRISPER/CAS9 system and replacing our gene with GFP. After performing a double transfection using the guide RNA and the donor...

13 February 2017 211 6 View

Problem with phosphoprotein detection? BSA vs Milk and TBST vs PBS??

We have been having problems with high background in our western blots for phospho-proteins even though we are using BSA. We found that certian lots of BSA give high background. What are your...

04 February 2016 4,148 2 View

How do you select for single and double knockout in the Crisper/CAS9?

We are planning a gene knockout experiment using origene gene specific crisper knockout. We were wondering of the best way to select for double knockout without doing southern blot? will FISH do...

23 May 2015 1,039 3 View

How to calculate protein concentration in a competitive ELISA?

I have done a competitive ELISA for the first time and I am not sure about the way to calculate the concentrations? I did a log transformation of the data to generate the standard curve? Is this...

16 January 2014 8,394 3 View

Which one is better for gene expression quantification, Branched DNA or Real time PCR?

In biology, a branched DNA assay is a signal amplification assay (as opposed to a target amplification assay) that is used to detect nucleic acid molecules. I have been asked about which is...

26 September 2013 5,560 45 View

Any recommendations for 3T3L1 differentiation?

I am trying to differentiate 3T3L1 cells into adipocytes, any helpful suggestions?

24 September 2013 741 8 View

Does anyone know if there is a commercial source for SuperSILAC protein mix?

I was wondering if there is a commercial source for super silac protein mix that are digested and ready made for use in MS.

16 September 2013 10,035 2 View

Does anyone have Orbitrap Fusion?

The Thermo ScientificTM Orbitrap FusionTM TribridTM mass spectrometer combines the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture that delivers...

03 September 2013 6,876 2 View

Does anyone have recommendation for THP1 transfection?

THP1 transfection

03 September 2013 7,513 5 View

Does anyone have experience with using DNA synthesis for cloning?

I was interested in using synthesized DNA for cloning. I am still used to the PCR based cloning. Any suggestions or recomendations before starting this process? Any good companies?

03 September 2013 2,635 11 View

Does anyone have experience with the Lipofectamine 2000 toxicity?

Do you have a toxicity problem with lipofectamine 2000? wht type of cells and what do yuo do to solve it?

03 September 2013 3,437 11 View