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Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....
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I would like to specify my question regarding the transformation of plasmid DNA into F graminearum protoplast. I am using the following reaction mixture (30%PEG 50ul, 200ul protoplast, and 20ug of...
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