Mit Joshi

5 Questions 4 Answers 0 Followers

Questions related from Mit Joshi

3T3L1 pre-adipocytes detached after 4 passages - What should I do?

I am working on a 3T3L1 cell line. I received my cell line with a passage number of 7. Cells were attached until the passage number reached 10. However, after that, cells began detaching from the...

12 February 2024 4,041 0 View

I am differentiating 3T3-L1 cells. Can someone tell me if there are lipid droplets present?

I am currently differentiating 3T3-L1 cells and I am uncertain about the presence of lipid droplets. However, before conducting the Oil Red O staining, I would like to know if there are already...

28 June 2023 388 4 View

How much concentration of RNA is required to synthesize cDNA and Real-Time PCR?

My concentration was 123.5 ng/uL 260/280 Ratio - 1.725 260/230 Ratio - 0.401 Can I synthesize cDNA from the above RNA? Need suggestions please.

21 April 2023 7,064 4 View

Can someone tell me what kind of cells or muscle are stained with red color in the picture?

Skeletal muscles are stained with the blue color but other half of the picture was stained with red color. Can someone tell me what kind of tissue or cell is it?

13 January 2023 2,921 3 View

Can anyone tell me which kind of contamination (fungal, yeast or bacterial) is this in cell culture flask?????

Can anyone tell me is this a contamination or just a cell changing its morphology?????

23 May 2022 6,844 4 View