Mishael al Bakar

3 Questions 7 Answers 0 Followers

Questions related from Mishael al Bakar

What is the use of an ori in mammalian cells?

I am trying to understand the origin of replication "ori" of a plasmid and its uses in mammalian cells. Does the ori used to make copies of the plasmid in mammalian cells? or you will get what...

13 April 2022 5,499 4 View

Using the spectrophotometer what wavelength should I use to read a sample of E.coli in a violet-bluish media?

Hey all, My question is about the spectrophotometer. I am trying to read the % T of E.coli in MaCckonkey broth which has the color violet to Blue. I am asking at what wavelength should I be...

30 October 2020 366 1 View

Does anyone know why dCas9 localize in the cytoplasm even with 2 NLS tagged in the c-terminal?

My knockdown wasn't successful in HEK293 because most of the dCas9 are found in the cytoplasm instead of the nucleus. I need to know what could be the reasons that cause dCas9 to localize in...

30 July 2018 346 10 View