Miriam Marschall

3 Questions 3 Answers 0 Followers

Questions related from Miriam Marschall

How you clean a protein from its antibody?

I've sorted my transfected cells with a primary antibody aginst the protein which was build in transfected cells. For sorting I additionally use a second antibody (anti-rabbit-FITC). Now I want to...

07 July 2016 3,206 9 View

How can I clone CFP in an mammalian expression vector?

I want to clone CFP as an control in an mammalian expression vector (pCI). Now my questions: how will I get the PCR-Produkt for cloning (of which organism or can I buy it) and it's possible to...

11 November 2015 1,520 3 View

What should I do if the forward primer binds at two sites?

I wanna do a PCR with a forward and a reverse primer. Now I have the problem the forward primer is binding at two sites of the DNA. I can't change the forward primer because of my enzyme of...

07 July 2015 5,919 9 View