Minnu Jayapal

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Questions related from Minnu Jayapal

I have transiently transfected 67NRs for knockdown of GOI & tried puromycin selection, but unable to observe cell death even at 10ug/ml of puromycin?

I have transiently transfected 67NRs, seeded in a 96 well plate at a seeding density of 15000 per well, using Lipofectamine 3000. 24 hours after transfection, I observed that there are atleast 50%...

30 January 2024 9,357 0 View

Is there any tool to design shRNA unique for isoforms?

I tried generating shRNA unique isoforms. I searched broad institute, and it gave 4 sequences. But when I did BLAST sequences from Broad institute it came up with 100% similarity for 2 isoforms,...

24 January 2023 9,170 0 View

Am currently using xCELLIgence assay to look at the adhesion of cells when treated with different drugs but the AI of the control varies why is it?

The controls in different cradles of the xcelligence setup varies much . But this trend was seen especially in the cradle number 2 in two different experiments. What could be the reason for...

02 November 2018 5,528 2 View

In migration assays what is the significance of using carbonated transwell inserts?

I have to measure the cell invasion and migration using a traditional boyden chamber style fixed endpoint transwell assay, while going through the previous research papers, I have seen that the...

07 September 2018 3,254 2 View