Minal Bhalerao

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Questions related from Minal Bhalerao

What could be a good destaining solution when observing crystal violet stained biofilm?

I am working with Salmonella Typhimurium and performing biofilm formation experiment. Referred some articles where destaining solution were used as follows: 1) Ethanol:acetone (80:20) 2) 30%...

19 January 2023 6,340 3 View

Any leads for identification of Pseudomonas syringae from a leaf or soil sample?

We are looking for the presence of Pseudomonas syringae in an infected leaf by the same pathogen. We tried isolating the pathogen in a selective medium named King's B medium. This pathogen forms...

26 May 2022 9,977 1 View

What is an appropriate protocol to process a bacterial culture before using for growth assay in minimal medium?

1) Using a secondary culture for inoculation in a minimal medium. 2) Washing the overnight culture cells and then using them for inoculation in a minimal medium. 3) Using directly the overnight...

13 January 2022 7,412 3 View

Preparing a solution of 99.5% Acetaldehyde

What could be the best procedure to prepare a solution of 99.5% acetaldehyde? Note: Acetaldehyde is a volatile chemical having a boiling point of 20.1 degrees Celcius.

01 January 1970 6,624 0 View