11 Questions 6 Answers 0 Followers
Questions related from Min Kang
Hi there, I am currently reading a document from applied biosystems called "Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR"....
23 February 2018 9,020 5 View
Why can't we use TAE buffer with TBE gel (and vice versa?) during gel electrophoresis? What's the chemistry behind it?
09 September 2017 4,009 4 View
Hi there, I am getting different heights for the amplification curves when I set up my standard curve. I usually get the first curve always lower than the latter curves. (graph 1: 98% efficiency,...
08 August 2017 7,961 3 View
Hi there, I am doing a qPCR experiment and I am going to compare cytokine stimulated cells vs. unstimulated cells' gene expression levels. I need around 10 million cells (1 X 10^7) for RNA...
08 August 2017 4,295 3 View
04 August 2017 7,441 2 View
03 August 2017 7,607 2 View
Hi there, I am seeing two peaks in melting curve for qPCR. My negative control shows me that it's not primer dimer nor genomic DNA. The peaks are similar in height and they are adjacent to each...
30 July 2017 6,255 6 View
Hi there, I have been trying to optimize primers on qPCR. Their Tm is 53.7 and 54.8. I am using FastStart Essential DNA Green Master (Roche) which has the following qPCR protocol: 1....
23 July 2017 1,788 5 View
Hi there, I was wondering if the cells have the "spread-out" spider web looking morphology, does this mean that the cells are senescencing? Do they also have "bubbles" in their cytoplasm? Thank you,
11 July 2017 807 3 View
07 July 2017 1,041 7 View
07 July 2017 4,189 3 View
