Min Joo Kim

3 Questions 1 Answers 0 Followers

Questions related from Min Joo Kim

How to deal with organoids splitting early in differentiation?

I'm using the Sasai protocol (https://www.pnas.org/content/110/50/20284#ref-16) for generate brain organoids. I'm currently at day 12 where the organoids are still in their 96 v-bottom well...

26 October 2021 8,082 0 View

Can you use MTS assay with cells cultured in media containing phenol red?

I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...

04 February 2021 9,936 2 View

Why did my media become cloudy and congealed upon incubation during iPSC > HPC > Microglia differentiation following simplified protocol?

I am trying to differentiate microglia following the simiplied protocol from the Blurton-Jones lab (Development and validation of a simplified method to generat... ). On the second step of the...

26 October 2020 7,674 3 View