Mikel Arana

2 Questions 4 Answers 0 Followers

Questions related from Mikel Arana

Which Positive Control Is Optimal for DCFDA-Based ROS Measurement?

I am optimizing my DCFDA assay in prostate cancer cell lines and have two main questions regarding positive controls: Should hydrogen peroxide (H₂O₂) be added during or after DCFDA incubation for...

15 March 2025 2,564 2 View

ROS assay quantification standarized protocol?

Hello everyone, I am conducting an assay to measure reactive oxygen species (ROS) using DCFDA (Sigma #D688) in prostate cancer cell lines and have some questions regarding the protocol. Adherent...

14 March 2025 7,005 4 View