Miguel Abreu

3 Questions 1 Answers 0 Followers

Questions related from Miguel Abreu

Does anyone know how to fix a microdilution broth assay with cloudy samples?

Hey everyone, I'm currently trying to determine the MIC of some samples, against Listeria, but I cannot do it by visual observation of button presence/absence since the samples are highly...

30 August 2014 5,652 3 View

Incomplete SDS-PAGE separation, what is the problem?

Sometimes when I run my Glycine SDS-PAGE, the separation of proteins (and ladder, also!) stops by the middle of the gel. This is getting more recurrent, since my last 2 gels have had this problem....

15 April 2014 2,605 3 View

How can I get a good gel in electrophoresis of milk and fermented whey proteins?

I'm currently trying to analyse the milk and fermented whey's protein profile using SDS-PAGE. I've already tried with Tris-Glycine and Tris-Tricine, but I always keep getting a smeared and poor...

27 February 2014 6,344 3 View