Michelle G Pitts

5 Questions 8 Answers 0 Followers

Questions related from Michelle G Pitts

Simple succinate dehydrogenase assay?

Can anyone point me toward a simple assay for either succinate or succinate dehydrogenase? I'm looking for something that I can learn and then work with an undergraduate student within a limited...

08 August 2018 8,747 2 View

Has anyone had trouble with using cytochalasin D in neutrophil experiments?

Hi all! I'm looking to decrease bacterial uptake by PMNs and I've been using Cytochalasin D with limited success. The bacteria are opsonized, so as far as I know this is driving a CR3-mediated...

07 July 2017 2,509 1 View

What is the best condition to store aliquots of dihydrorhodamine 123?

If I resuspend dihydrorhodamine 123 in EtOH, is it best to store aliquots in -20 or in liquid N2? Also I've seen one recommendation not to use a reconstituted aliquot beyond 1 month. What's the...

11 November 2016 2,640 4 View

Can anyone ID these cells by FACS?

This is from bone marrow after a gradient to deplete non-PMNs. I attached a scatter plot showing a group in the upper right accounting for about 6% of my total. These cells are CD11b+, Ly6G very...

08 August 2016 2,633 11 View

Does anyone know why HBSS + 0.1-1.0% gelatin is commonly used in neutrophil isolation and bacterial opsonization protocols?

I'm planning to isolate BM PMNs and use normal mouse serum to opsonize Listeria. I see in older papers with other bacteria that HBSS with gelatin has been used. I'm not readily finding a reason...

07 July 2016 4,739 4 View