Michelle Klesse

3 Questions 3 Answers 0 Followers

Questions related from Michelle Klesse

Does anyone have experience with the use of either recombinant murine or human MPO in in vitro cultures of murine primary cells?

I wish to investigate the effect of (recombinant) MPO on in vitro cultured murine primary cells, such as T cells. MPO is mostly known to affect cells through its enzymatic activity, but has also...

25 March 2022 1,251 0 View

What are optimal centrifugation settings for murine eosinophils?

Hi everyone! I am currently trying to differentiate and culture murine eosinophils from bone marrow cells (with FLT3-L, SCF, and IL-5) according to Dyer et al., 2008. After 18 days in culture I...

23 March 2020 5,895 3 View

What is the optimal pretreatment for fluorescence in situ hybridization on human skin samples?

I am currently trying to do FISH on human skin biopsies that have been routinely fixed in formalin and embedded in paraffin. I am using the CEN X/Yq12 probes from ZytoVision and their ZytoLight...

11 October 2019 5,943 4 View