Michel Wehbe

2 Questions 3 Answers 0 Followers

Questions related from Michel Wehbe

Does you have a working protocol for TCID50 and PFU (plaque forming unit, using methylcellulose + MEM10x) for an enterovirus using HeLa cells?

Hello, Currently I am trying with HeLa cells but in both cases I’m having problems with cell viability, so my cells are dying before I can see final results (seeding density for TCID50 96 wells...

01 January 2015 3,756 1 View

Can you help me with reverse transcription of full length Coxsackievirus B3 genome?

It's been more than 2 weeks that i'm trying to have a full length cDNA copy of Coxsackievirus but have only fragments. I tried to use oligo dT and specific primers on the 3'End of the viral...

07 July 2014 4,292 0 View