Michael J Shreve

3 Questions 7 Answers 0 Followers

Questions related from Michael J Shreve

What is the best method for calculating percent removals from censored data (i.e. data reported as 'not-detected')?

I've sampled the influent and effluent of six wastewater treatment plants and analyzed for a large number of trace organic chemicals. There are many instances where the commercial lab conducting...

09 September 2017 4,454 7 View

How can I reduce non-specific product in PCR amplification of environmental gDNA AND proper annealing temperature for 515f-806r primer set?

I am currently optimizing PCR for amplification of the ITS2 region for fungi and V4 region for archaea/bacteria from environmental gDNA. This amplification is being done using degenerate primers...

01 December 2016 9,551 3 View

What is an appropriate biological replicate for sampling biomass in a wastwater treatment system?

With regard to amplicon-based metagenomics using next generation sequencing, would multiple samples collected from the same well-mixed wastewater treatment basin approximately 10 minutes apart be...

21 July 2016 6,598 2 View