Michael J Shreve

2 Questions 5 Answers 0 Followers

Questions related from Michael J Shreve

What is the best method for calculating percent removals from censored data (i.e. data reported as 'not-detected')?

I've sampled the influent and effluent of six wastewater treatment plants and analyzed for a large number of trace organic chemicals. There are many instances where the commercial lab conducting...

09 September 2017 4,235 7 View

How can I reduce non-specific product in PCR amplification of environmental gDNA AND proper annealing temperature for 515f-806r primer set?

I am currently optimizing PCR for amplification of the ITS2 region for fungi and V4 region for archaea/bacteria from environmental gDNA. This amplification is being done using degenerate primers...

01 December 2016 9,324 3 View