Michael Sandel

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Questions related from Michael Sandel

Why would ancient paralogs become syntenic within a single chromosome?

During whole genome duplication, thousands of genes are duplicated, and many are lost. Among those that are retained, there is no obvious pattern to the genomic location of descendant paralogs. In...

07 September 2024 6,343 0 View

Which form/type of epigenetic modification is most closely associated with transgenerational effects?

Cytosine methylation, histone acetylation, etc. represent distinct epigenetic mechanisms of gene regulation. Which example is most likely to account for transgenerational effects, such as...

01 June 2024 4,680 3 View

Can you use a syringe to pull tissue digest through a Qiagen spin column?

Qiagen DNeasy/Qiaprep spin column protocols call for either a centrifuge or vacuum to pull the tissue digest through the spin column membrane. In a pinch, could a syringe be used to create the...

13 May 2023 6,809 1 View

Does nested PCR reduce sensitivity for detecting heteroplasmy and/or heterozygosity?

My lab has designed a nested PCR assay (~500bp) for a bacterial plasmid (pMUM) for use in environmental detection. The assay works well, but in an effort to replicate our findings we encountered...

22 December 2021 9,416 2 View

Is it possible to quantify cellular mitochondria using a light microscope, or are more expensive technologies needed?

I would like to visually compare mitochondrial abundance/density across various tissue types. Can this be done with normal histology and a light microscope?

12 June 2018 5,813 5 View

Is the Veriti thermal cycler a durable machine?

I am setting up my first genetics lab, which will be used for training students and conducting research. I grew up on the ABI 9700, and I am sold on the reliability of this product (compared to...

04 February 2015 1,051 3 View