Michael Bitar

4 Questions 4 Answers 0 Followers

Questions related from Michael Bitar

Is the CFSE staining with PerCp.Cy5.5 compatible?

Hallo my colleagues, I staind the PBMCs with CFSE for Proliferation and with CD3 PerCpCy5.5, i noticed that CFSE staining alone overlapped with CD3 PerCpCy5 , where i could not do the...

22 July 2020 177 3 View

I detect NFKB in the T cell after stimulation?

I try to detect pNFKB after Stimulation with PMA+ ionomycin (400nM+1µM) respectively(15 min ). some People were detected but other not , and it was not Signal of p.NFKB at These . has anyone...

05 October 2016 4,800 1 View

Why is percent of T-bet always low, not more than 4%, although CXCR3 +CCR6- CD4+ is 25%?

I try to establishment a Panel TH1 cells by surface antigen CXCR3 + CCR6- and transcription Factor T-bet. But the problem is always the percentage of T-bet low(3-4%)I hope someone can help me

01 December 2015 9,883 9 View

Could I use anti CD3/CD28 to stimulate T cells and measure intracellular cytokines ?

i want use CD3/CD28 to measurement intracellular cytokines someone has Protcol.please

04 November 2015 7,614 7 View