Michael Bitar

6 Questions 6 Answers 0 Followers

Questions related from Michael Bitar

Is the CFSE staining with PerCp.Cy5.5 compatible?

Hallo my colleagues, I staind the PBMCs with CFSE for Proliferation and with CD3 PerCpCy5.5, i noticed that CFSE staining alone overlapped with CD3 PerCpCy5 , where i could not do the...

22 July 2020 216 3 View

Measurement of pNFkB -NFATc1 after stimulation with PHA by Flow cytometry?

i try to measure pNFkB and NFATc1 after the Stimulation with PHA for 30-60-90-120min , by Flow cytometry , but It does not work and it does not find a Signal of phosphorylation . anyone please...

26 October 2016 4,009 3 View

I detect NFKB in the T cell after stimulation?

I try to detect pNFKB after Stimulation with PMA+ ionomycin (400nM+1µM) respectively(15 min ). some People were detected but other not , and it was not Signal of p.NFKB at These . has anyone...

05 October 2016 4,823 1 View

I search of optimal concentration of LPS or PMA to stimulate NFkB in T cells in 200µl whool blood ?

i try to get optimal Stimulation of NFkB in Tcells with LPS or PMA OR PMA with ionomycin has anyone a advice or Information thanks

13 April 2016 3,076 2 View

Why is percent of T-bet always low, not more than 4%, although CXCR3 +CCR6- CD4+ is 25%?

I try to establishment a Panel TH1 cells by surface antigen CXCR3 + CCR6- and transcription Factor T-bet. But the problem is always the percentage of T-bet low(3-4%)I hope someone can help me

01 December 2015 9,903 9 View

Could I use anti CD3/CD28 to stimulate T cells and measure intracellular cytokines ?

i want use CD3/CD28 to measurement intracellular cytokines someone has Protcol.please

04 November 2015 7,636 7 View