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Questions related from Michael Attwaters
I have mutated a gene in the zebrafish and believe there is a phenotype in homozygous mutants, albeit a subtle one. I am able to pick out mutants at ~4--5 dpf based on a slight bend in their...
03 July 2020 1,832 3 View
I have measured NAD+ concentrations in tissue and get a value of approximately 2 uM/1 mg. I want to compare this value to literature where the values are commonly reported as nmol/mg. How can I...
19 January 2019 3,677 3 View
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19 January 2019 7,858 3 View
Has anyone measured the NAD/NADH content of whole zebrafish larvae? If so what quantity of NAD+ and NADH have you recorded? I can't find any papers that report such values. Cheers, Mike.
19 January 2019 4,851 0 View
I am attempting to make sgRNA but I am having problems with cloning my oligonucleotides into the BsaI-cut pDR274 vector. The problem is that I am left with many colonies containing the empty...
11 January 2019 5,180 2 View
