Mg Li

4 Questions 2 Answers 0 Followers

Questions related from Mg Li

Help needed with validation an ELISA PK Assay?

Hi Experts, I encountered difficulties during the validation of a mouse serum pharmacokinetic (PK) assay. While the recovery and %CV were consistently good for standards, I observed significant...

01 April 2024 651 0 View

How long time is best for TMB development in PK assay development?

Hi PK assay experts, have one question about TMB development time in PK assay development. I have monitor OD650 for my PK assay. after TMB reaction 30min, the OD650 was increasing very slowly....

10 March 2024 6,488 0 View

Coating condition for ELISA?

Hello ELISA experts, I met a weird ELISA results recently. The background is not repeatable and my coating protein is a recombinant protein not a antibody. any suggestions for coating...

11 December 2023 5,528 0 View

Background signal changing?

Hi ELISA experts, I need your help to trouble shooting my background signal keep changing. I have run the same ELISA about 2 weeks for anti-drug antibody detection assay development. I noticed...

18 November 2023 9,241 3 View