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Questions related from Meryem Köse
I take a portion of a colony from an E. coli streaked plate and inoculate it into 50 ml of LB medium. It is incubated in a shaking incubator for 24 hours. After 24 hours, 2 ml is transferred into...
08 July 2025 1,785 0 View
I want to create hypoxia with cocl2.6h20, should I subtract the molecular weight of sun or should I use the molecular weight of cocl26h2o?
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I expose lung cancer cells to hypoxia to study gene expression. The cells were kept in a hypoxic environment for 6 hours, and after 6 hours, I lifted the cells using trypsin and left them in the...
27 April 2025 4,610 1 View
When I do RT-PCR, I use cDNA at a ratio of 1:100.18S value is good, but beta-actin ct value is 35.It also gives abnormal values in genes I use other than housekeeping.Can I run the cDNA in the...
30 January 2025 8,174 1 View
Hello, I am going to transfect A549 cells by creating a mutant form of a protein.then I will create a spheroid model of these cells.for the spheroid model I am thinking of coating 24 well plates...
18 December 2024 5,004 2 View
I have atezolizumab with a concentration of 59 mg/ml. I want to prepare this drug with DMSO to be 10mM. How much DMSO should I add? Also, how many concentrations should I give to A549 and Calu-1...
19 August 2024 4,306 1 View
I am trying to obtain protein from paraffin tissue. I expose it to xylene twice and incubate it at 56 degrees. My protein concentration is low. What do you think could be the reason? I use the...
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