Meriem Hamdi

5 Questions 3 Answers 0 Followers

Questions related from Meriem Hamdi

Fetal Bovine Serum, exosome-depleted heat inactivation?

Hi, everyone i am going to use FCS- depleted exosomes From Thermofisher, for primary cell culture of bovine oviduct epithelial cells , and i am wondering if i need to inactivate it befoe its use

12 October 2020 6,107 4 View

Can I prepare Optiprep density gradient with PBS?

I am trying to isolate Extracellular vesicles (EVs) from follicular fluid using the Optiprep density gradient (ODG), but i am wondering if i can just use PBS 1X instead of sucrose 25mM to prepare...

12 September 2019 1,902 5 View

What is the role of the application of Aldehyde/Sulfate Latex Beads (invitrogen) in the flow cytometry assay ?

I am trying to purificate Extracellular vesicles (EV) from BOEC conditioned media by coloumn of chromatography, then i want to charachertize the purified EV by flow cytometry. However i have seen...

15 October 2018 4,717 4 View

What to choose as a calibrator? - When looking at basal gene expression (Rt-qPCR)?

What to choose as a calibrator? - When looking at basal gene expression (Rt-qPCR)

19 October 2017 6,944 3 View

Does PCR efficiency colse to 1 is the same as 100%?

When we speak about PCR efficiency to be close to 1 does it mean the same as 100%. I want to perform RT-qPCR with bovine blastocyst to analysis some genes related with metabolism. Does a pool of...

02 October 2017 9,976 5 View