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Questions related from Meriem Hamdi
Hi, everyone i am going to use FCS- depleted exosomes From Thermofisher, for primary cell culture of bovine oviduct epithelial cells , and i am wondering if i need to inactivate it befoe its use
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I am trying to isolate Extracellular vesicles (EVs) from follicular fluid using the Optiprep density gradient (ODG), but i am wondering if i can just use PBS 1X instead of sucrose 25mM to prepare...
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I am trying to purificate Extracellular vesicles (EV) from BOEC conditioned media by coloumn of chromatography, then i want to charachertize the purified EV by flow cytometry. However i have seen...
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What to choose as a calibrator? - When looking at basal gene expression (Rt-qPCR)
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When we speak about PCR efficiency to be close to 1 does it mean the same as 100%. I want to perform RT-qPCR with bovine blastocyst to analysis some genes related with metabolism. Does a pool of...
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