Meng Huang

4 Questions 4 Answers 0 Followers

Questions related from Meng Huang

What is the function of ubiquitously expressed promoter for target gene expression?

What's the advantage of this promoter?

11 November 2018 8,339 1 View

Is that OK to keep PCR machine in 4C keeping sample for over night?

I always saw some people use PCR machine and keep their sample inside at 4C or some other low temperatures for overnight. The result is water accumulated around the matel plate. Is that influence...

07 July 2018 1,258 2 View

What is a depletion mechanism in PEG induced protein precipitation? Is there any model to show it?

I'm trying to understand more about nucleation work, since I have no background about physic chemistry or even not much chemistry. But this concept came out many times during my way to know more...

06 June 2018 1,677 3 View

Why my target protein show less molecular weight after run SDS-Page?

I expressed my recombinant protein in E.coli, BL21 (DE3) with a MBP fusion protein. But after cut by protease to remove the MBP part, I found my target protein size is less than I expected, but...

06 June 2018 5,230 3 View