Mehwish Iftikhar

3 Questions 10 Answers 0 Followers

Questions related from Mehwish Iftikhar

Separation of protein via Akta system gave broad peak during elution. I wonder is it normal to obtain broad peak ?

Greetings.. I am using Akta Pure system for my protein purification. I have optimized buffer system for protein elution. However, this time I got broad peak while eluting sample. For...

25 December 2020 545 7 View

While running 15% SDS gel, I have noticed some blurred blue coloration above sample protein. What it could be?

While running 15% SDS gel, I have noticed some blurred blue coloration above sample protein. I have attached figure for the convenience. Suggestion and answers in this regard will be highly...

29 October 2020 2,967 1 View

Desired membrane protein band isn't aligned with marker in SDS-PAGE gel. What could be the possible reason?

I am having problems regarding to protein separation via SDS-PAGE. As a newbie in this field, I have tried to optimize the conditions for protein induction. My protein size is in 20-25KDa range...

04 September 2020 8,349 11 View