Megha Sah

4 Questions 4 Answers 0 Followers

Questions related from Megha Sah

Any advice on live imaging analysis using Z stack?

I have recorded a video using fluorescence signaling from live (and unfortunately moving) cells and saved it as a stack in ImageJ. I am looking for a way to select a region of interest that is...

07 July 2015 4,405 4 View

What does infection of neurons with a mutant form of protein do to the endogenous protein level?

I want to look at the effect of a mutant form of GEF (guanine nucleotide exchange factor) on the resultant downstream small GTPase activation in neuronal cultures. To do so, I am planning on...

07 October 2014 8,732 2 View

Why is my protein of interest getting stuck at the stacking and separating gel interface?

180 kDa protein is getting stuck at the stacking gel and separating gel interface. Stacking gel-3.5% Separating gel-12.5% (this percentage is chosen because we run a 18kDa protein along with the...

15 February 2014 5,323 5 View

How do you prepare a 0.1M Cadmium chloride solution for treating neuronal cultures?

I am running into a constant problem of the Cadmium chloride precipitating out of solution if I try to make up a solution with dPBS or aCSF(due to the presence of divalent ions in these...

07 January 2014 4,588 2 View