Megan Mitchell

3 Questions 4 Answers 0 Followers

Questions related from Megan Mitchell

Is there a way to simultaneously visualize DNA and protein following electrophoresis?

I am currently optimizing the conjugation of a DNA oligo sequence to a DBCO activated antibody and am looking for a method which could allow me to simultaneously visualize the amount of DNA which...

05 May 2019 2,206 3 View

Does anyone have experience with methods to overcome fluorescent overlap between Doxorubicin and mCherry?

I am looking to assess the metabolic control of breast epithelial cancer cells in cancer-associated fibroblasts (CAFs) and the subsequent effect of this on doxorubicin chemoresistance using an in...

01 January 2017 6,611 1 View

How do I go about setting up an accurate in vitro model of normoglycaemia in MDA-MB-231 cells?

Currently we culture our MDA-MB-231 cancer cells in DMEM (containing 25mM glucose) with 10% FBS + 1% PenStrep. I am wanting to assess the effects of different glucose concentrations (25 mM and 5...

03 March 2015 4,332 4 View