Meenal Chauhan

7 Questions 10 Answers 0 Followers

Questions related from Meenal Chauhan

Whare are the alternatives of 1,2-dichloroethane to dissolve periodide crystals during glycine betaine estimation?

During Glycine betaine estimation, the periodide crystals are to be dissolved in 1,2-dichloroethane. However, My current lab do not have a fume hood, therefore, I am looking for a safer, but...

2 weeks ago 6,446 0 View

What is the limit of ORP at which anaerobic bacteria can survive?

I am trying to cultivate anaerobic cultures. Currently, the ORP is -400 mV, I was wondering if it could go even further such as -600 or -1000 mV. Do anaeorbes can still survive at this ORP values?...

05 December 2024 6,758 1 View

Which expression vector is suitable for transfection or top deliver a gene encoding a specific protein in an anerobic bacterial culture?

I want to deliver a gene which codes for a protein of interest to an anerobic bacterial culture, but there is a very specific condition that the protein production should be either induced...

24 October 2024 4,270 5 View

How to estimate a protien's size using Chimera or PyMol? how correct this estimated can be?

I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...

22 July 2024 5,932 3 View

Brodening of an an elution peak can be a possible cause of target protein's different conformations?

I observed a broadening of an elution peak in Gel filteration and my target protein elutes within this broaden peak. I am wondering about the possible cause of it. literature mostly says it...

22 July 2024 1,681 5 View

How to bring baseline to zero for an absorbance data for chromatogram?

I forgot to autozero during the run (Size exclusion chromatography.) and later i realised i forgot to do that and the baseline was not zero but below zero (and in some cases it above zero). I...

16 July 2024 5,854 6 View

How to identify a monomeric protein species of the target without running the molecular weight standard on SEC?

I was using Superose6 10/300 Gl column for purification of my target protein. the sample consists of various oligomeric state and i would like to identify the approximate elution volume where my...

16 July 2024 5,272 2 View