Md Thoufic Anam Azad

3 Questions 8 Answers 0 Followers

Questions related from Md Thoufic Anam Azad

How can I delete 200 bp from my plasmid ? Please advice?

I have inserted a gene approximately 2000bp for protein expression in pCS2+MT vector. But now I need to delete 200bp from that gene. If I delete it. it will remain inframe.

02 February 2016 7,302 12 View

What are the causes of dot and smearing band of digested plasmid?

I am digesting pCS2+MT plasmid with Xhol-Xba1 double digestion. Previously it was ok but now the gel image like dot and smearing ? So ligation failure !　Could you please tell me the reason? I am...

04 April 2015 7,603 4 View

How we can irradiate at low level to BHK, HEK cells during incubation?

I want to irradiate cells to see some chances at mRNA. How can I irradiate cells keeping them viable during incubation?

12 December 2014 651 1 View