Md Abu Siddique

6 Questions 8 Answers 0 Followers

Questions related from Md Abu Siddique

How to Perform and calculate Serial Dilution in a 96 well efficiently ?

I was about to add an enzalutamide drug and check IC50 and later on wanted to do MTT assay in a 96 well plate. But I found the calculation of serial dilution little bit hectic. Is there any easy...

17 June 2022 4,804 0 View

I need to treat my Prostate cancer lines with EGF in a 6 well plate. What is the amount i need to apply ?

Right now I am serum starving my cell lines. What is the amount of EGF I need to apply in per well of 6 well plate. What is the calculation for finding right amount of EGF? i have already...

29 March 2022 3,972 1 View

What is ratio of ripa buffer based on number of cell count ? And how much protinase and phosphatase K inhibitor i need to add?

I am planning to seed 2x10e5 cells per well in a 6 well plate. How much ripa buffer i need to add ? What will be the amount of protinase and phosphatase k inbitor cocktail mix to add with ripa buffer?

16 March 2022 7,916 5 View

Which bead is good for immunoprecipitation?

I am using abcam protocol for IP. They recommend G-coupled Sepharose beads . Will it be good? Please recommend a proper bead for IP

11 March 2022 8,435 2 View

What concentration of protease inhibitor and phosphatase inhibitor for six well plate?

I have to add protease inhibitor and phosphatase inhibitor during cell lysis in a six well plate. How can i calculate the amount of protease and phosphatase inhibitor amount ?

08 March 2022 312 1 View

I want to develop the Phosphorylation assay of a transcription factor. What is the best way to find out the phosphorylation site ?

Need to establish the assay development of phosphorylation of a transcription factor that contributes to prostate cancer. I want to develop the assay manually rather than using any Kit.

01 February 2022 741 12 View