Md. Sakib Hossen

4 Questions 4 Answers 0 Followers

Questions related from Md. Sakib Hossen

What is the best ChIP-qPCR kit and antibody for studying thyroid hormone receptor beta (THRB) in HepG2 cells?

I'm currently performing ChIP-qPCR using the ChIP-IT® Express Kit (Active Motif) in parental HepG2 cells to study THRB binding. However, I suspect that the antibodies I’ve used may not be working...

13 April 2025 251 0 View

How Can I Optimize Sonication, DNA Precipitation, and Nuclei Release for HepG2 Cells Using Bioruptor® Pico?

Sonication: I'm currently optimizing the sonication conditions for shearing chromatin in HepG2 cells using the Bioruptor® Pico for downstream applications like ChIP-qPCR. While I've tried various...

18 August 2024 5,052 0 View

How can I Visualize protein-protein interactions with LIGPLOT ?

0 📷 I did protein protein docking through clus Pro then I am trying to see the interaction through ligplot but after putting the docking results it's not showing anything its showing "no...

27 July 2022 3,913 7 View

How can I make a phylogenetic tree (by using Mega or any other software) which resolution will be at least 600 dpi?

Recently I made a phylogenetic tree by using Mega. But, to fulfill submission criteria in a journal, its resolution might be 600 dpi (file format will be TIF). I was also tried some online dpi...

13 February 2018 5,201 0 View