Md. Saddam Hussain

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Questions related from Md. Saddam Hussain

Any divalent metal ion chelator that selectively chelates Manganese over Magnesium?

Hi, Research Community I am working on a two-step enzymatic reaction, wherein 1st step requires Mn2+ while 2nd step needs Mg2+ supplementation for optimum cleavage of substrates. The presence of...

01 October 2024 7,801 3 View

Why does a recombinant protein (6xHis tagged) not eluting out after binding to Ni-NTA-agarose resin?

Hello everyone, I am struggling with the purification of a recombinant protein (6xHis tagged). The overexpressed protein binds to Ni-NTA-agarose beads adequately. However, it is not eluting out...

29 October 2022 5,082 5 View

Is there any report on leaky scanning of RNA leading to co-translation of two proteins from a single RNA in prokaryotes?

Heterologous expression of a bacterial gene in BL21 cell is generating two protein of different size: one of full length and another one of smaller size. The possibility of fragmentation...

09 October 2019 1,364 3 View