Md. Atiqur Rahman

3 Questions 15 Answers 0 Followers

Questions related from Md. Atiqur Rahman

Can anyone suggest the best way to transfect cloned plasmid vector into a mammalian cell line?

Hi everyone!I want to transfect my cloned plasmid vector into thyroid cancer cell lines (8505C, B-CPAP, K-1, MB-1 and BHT-101). Can anyone suggest me the best way to do the transfection and advise...

30 October 2014 417 7 View

How to clone a 59 bp shRNA into a 6990 bp vector? What are the things I need to be careful about to get a successful clone?

Currently I am cloning a 59 bp shRNA into a 6990 bp vector. The shRNA contains 5' phosphate. I did a double-digestion of the vector with Xho I and Hind III. Then I run a gel to confirm that the...

05 February 2014 834 21 View

How to separate fibroblast cells from carcinoma cells in primary cell culture?

Currently I'm doing primary cell culture of carcinoma by ex-plant method. In the culture, I observed fibroblast cells along with carcinoma cells and fibroblast cells are growing faster than the...

25 January 2014 4,947 14 View