Md Golam Kibria

3 Questions 2 Answers 0 Followers

Questions related from Md Golam Kibria

Why A431 cell are not detached from the cell culture plate after trypsinization?

For the first time, I am culturing the A431 cell. After trypsinization, I have noticed that almost all of the cells are not detached. For trypsinization, I used 0.5 trypsin 0.53 EDTA and kept the...

12 June 2020 3,405 3 View

Why Endotoxin assay is required for immunogenicity experiment in mice ?

Currently, I am doing an immunogenicity experiment in mice with recombinant protein purified from E.coli inclusion bodies. In this case, what is the importance of measuring the endotoxin in the...

09 October 2019 2,923 4 View

Best Procedure for Ion exchange column Wash?

Currently I am using strong cation exchange column (TOYOPEARL GigaCap S-650M) in HPLC. I want to know the cleaning procedure of this resin. Thank You.

06 November 2018 2,897 2 View