Md. Ahasan Ahamed

2 Questions 3 Answers 0 Followers

Questions related from Md. Ahasan Ahamed

In an RPA reaction, when should I add 5% DMSO?

I have to add 5% DMSO to reduce primer dimer in the RPA reaction. When Should I add it? Should I add during master mix making or after amplification, or after DNA purification?

12 February 2023 9,626 1 View

What are the reasons of this type of weird band in RPA reaction and how can I get did of it?

I am working in RPA for the first time. I followed the protocol to design the primer using the twistdx manual. I bought target, which was fragmented. I figured out the exact sequence by using a...

02 February 2023 5,533 4 View