Mazhar Hussain

6 Questions 6 Answers 0 Followers

Questions related from Mazhar Hussain

How to do CUT and TAG normalisation for EZH2 inhibited cells in H3K27me3 pulldown samples?

Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...

22 April 2024 3,166 0 View

Is there an alternative for Poly(dI.dC) to use in Electrophoretic Mobility Shift Assay?

I am planning to perform EMSA for protein-DNA interactions. In order to do so, I need something that blocks the non-specific DNA-protein interactions. People have used Poly(dI.dC) in their assays....

22 July 2021 4,563 0 View

Can you grow yeast on selective broth after transformation, before plating to selective agar plates?

I have been using Takara's Yeast Transformation System 2. Using the kit's protocol, I transformed Saccharomyces cerevisiae with plasmids that have auxotrophic selection (trp and leu) in them. I...

13 July 2021 3,695 4 View

How to decrease the shrinking of Wells in Hoefer SDS PAGE system?

Hello everybody, I have been using Hoefer SDS PAGE system. It gives fantastic bands. But the only problem with this system is that it's combs are open which causes it's wells to shrink down. It...

09 June 2016 6,902 6 View

How do I fit incidence data of epidemic to SEIR model?

Hello everyone, I am working on a mathematical model. I have weekly incidences of a virus epidemic. So, my proposed modeling approach is deterministic model using system of non-linear differential...

08 September 2015 5,463 4 View

How might I make sense of contour graphs in this article on Ebola?

Recently, I have been studying the paper "Estimating the basic reproductive ratio for the Ebola outbreak in Liberia and Sierra Leone", published in Infectious Diseases of Poverty. I came across...

05 August 2015 675 1 View