Maximilian Paradiz

5 Questions 6 Answers 0 Followers

Questions related from Maximilian Paradiz

Could this Cas9 anti-HIV strategy possibly work?

Dear RG friends, I have been looking at the recent failed attempts to remove HIV using CRISPR/Cas9, and I have been thinking about potential ways to solve the current problems with the technique....

05 August 2016 7,436 4 View

What is a good method to BLAST a protein using amino acid properties rather than their specific identity?

For example, I would like to BLAST for proteins containing a particular amino acid pattern, such as 'hydrophobic, positive, anything, hydrophobic, positive, anything....', rather than looking for...

01 August 2014 9,076 3 View

What method can I use for purification of a His-tagged synthetic peptide?

We want to order a 20-mer synthetic peptide. We are considering adding a His-tag to the 20-mer (making it a 26-mer) and ordering the crude product, and then purifying it ourselves using his-tag...

05 June 2014 7,144 4 View

How do you identify a specific enzyme in a library of mutants?

Lets say I want to make an enzyme that catalyzes the conversion of A into B, where neither A nor B affect the cell's viability in any significant way. I know that there is an enzyme "X" which...

04 August 2013 4,188 3 View

Is there a known genetic mutation/mechanism through which a given polypeptide can have its amino acid sequence inverted pre-translationally?

For example: A DNA sequence is translated into the sequence: N-MKTSTRFLDGYFPVAANK-C -Mutation- Which leads to the production of: N-(Met)KNAAVPFYGDLFRTSTKM-C The obvious problem is...

10 May 2013 3,367 1 View