Why does my DAPI(viablity dye) stains high DNA content cells for no apparent reasons in FACS?

Just for some context, I'm isolating non parenchymal cells with Pronase+Collagenase D, which is a well known protocol in my lab. I use DAPI as a viability dye in my FACS panel but I encounter some...

14 October 2021 2,461 2 View