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Hello, I am looking for an alternative for Seqeuncher since our license is running out and wanted to see what people's opinion are for Sanger sequence editing. I know there are a lot out there,...
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Hello All, I am working on trying to increase the rates of successful integration of a transgene in catfish using CRISPR HDR knock-in. Right now we are using microinjection on eggs shortly...
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My lab is embarking on using CRISPR coupled with HDR for transgenic insertion. We have been using CRISPR for gene knock-in for a couple of years now with good success rate (60-90%). However, we...
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I am working on developing donor constructs to be used for a transgenic project where we are using CRISPR coupled with HDR to insert our gene of interest in channel catfish. In the past we have...
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I want to test for integration of a transgene and potentially determine where the transgene is integrating. Does anyone with experience in this area have any suggestions for doing this (i.e....
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