How can I increase the proportion of CD4+ Cells (CEMT4 cell line) that I can infect in vitro using HIV-1 JRCSF?

My spinoculation protocol provides high titers of of virus at 24 and 48 hours by p24 ELISA. However, my proviral DNA qPCR data as well as p24 flow cytometry suggests that only ~5% of the cells are...

06 June 2019 2,959 4 View