Matthew Lakins

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Questions related from Matthew Lakins

Has anyone used the tumour cell line CT26.CL25?

CT26.CL25 is a carcinoma cell line derived from colon. This cell line has been stably transduced wtih the lacZ gene encoding the model tumor associated antigen (TAA), beta-galactosidase (beta-gal)...

22 September 2016 9,680 1 View

Any tips on how to get rid of (or at least lower) the amount of green autofluorescence in FFPE sections when staining for immunofluorescence?

Currently clearing with xylene and rehydration through lowering concentrations of ethanol (100%, 90%, 70%, H2O) and then carrying out Sodium Citrate antigen retrieval.We get a strong...

25 September 2014 8,014 4 View

How can I confirm the PC-3 cell line I have been working on is actually a PC-3 cell line?

You read about how people realise too late that their cell line has been contaminated by, for example, HeLa cells which outgrow and overtake the original cell line. So what is the best way of...

02 November 2012 10,025 11 View