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Based largely on the work of Morten Rasmussen we are interested in using the PAG promoter to drive expression of our transgene in glutamatergic neurons. Has anyone else used this as a promoter?...
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that will not run through agarose gel about 75% of the time. We've gone back to check the primers and adjusted
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I'm looking for vectors for DNA sequences larger than 5kB with low association of inflammation/necrosis around injection site and that will deliver and integrate material into (mammalian, en vivo)...
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