Matheus Macedo-Lima

4 Questions 4 Answers 0 Followers

Questions related from Matheus Macedo-Lima

Habituation/compensation after repeated muscimol microinjection?

We have found some evidence for behavioral habituation to repeated muscimol microinjections into the orbitofrontal cortex during an aversive operant procedural task. The effect seems to wear off...

09 September 2021 2,446 2 View

SCH-23390 solution at ~pH 7?

I've been trying to make a 7 mM solution of SCH-23390.HCl to be injected in the brain. Every attempt to regulate the pH after dissolving in water has made the drug precipitate (I've tried ACSF and...

12 June 2019 478 0 View

Unstable whole cell voltage clamp recordings at -70 mV with low Cl- Cs-gluconate internal: any ideas?

This is mostly a curiosity. In this experiment, I'm looking at putative GABAergic currents by recording at 0 mV using a low chloride (2.2 mM) Cs-Gluconate (120 mM) based internal solution...

07 May 2018 2,867 10 View

What causes the Cslow compensation error in PatchMaster?

We use PatchMaster with a HEKA EPC 10 amplifier and do whole cell recordings. In about 90% of the cases, when we go into whole cell mode and compensate for Cs, PatchMaster shows "wonky" values for...

21 October 2016 144 5 View