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Questions related from Matan Shelomi
I have annotated a genome successfully with PROKKA. I can upload the genome to NCBI, but I cannot upload the annotation. PROKKA does not produce files in the correct format, and I cannot find any...
30 December 2020 4,138 3 View
My lab is trying to do RT-PCR, by converting RNA to cDNA and then using the standard 16S bacterial primers and PCR conditions on it. Unfortunately all we get are giant streaks with no discernible...
03 December 2020 4,683 7 View
These kinds of tests are usually advertised as a means to identify a microbe to ones that are already known. I want to ask about using them as an efficient means of doing the necessary phenotypic...
15 April 2020 5,719 0 View
I have purchased a solid cellulase from Aspergillus niger (CAS Number 9012-54-8). I want to use it in solution as a positive control for CMC breakdown using agarose plate assays (incubate and use...
29 October 2019 4,843 2 View
Since fructose is also detected by reducing sugars tests, can I do a quantitative fructose test and subtract those results from the sugar test?
10 May 2019 3,813 3 View
I want to inventory the microbiome of small sources of water (say, the contents of a pitcher plant or a bromeliad, or even a puddle), using 16S rRNA. Most of the protocols and kits I found online...
27 June 2016 796 1 View
Low quality transcriptomes, contaminated data, publication restrictions, negative reviews on manuscript submissions that potentially compete with their work, suppression of other researchers......
01 January 1970 1,916 1 View
