Matan Shelomi

5 Questions 5 Answers 0 Followers

Questions related from Matan Shelomi

Once I annotate a genome with PROKKA, where do I uplaod it?

I have annotated a genome successfully with PROKKA. I can upload the genome to NCBI, but I cannot upload the annotation. PROKKA does not produce files in the correct format, and I cannot find any...

30 December 2020 4,072 3 View

Why is my cDNA PCR all streaky?

My lab is trying to do RT-PCR, by converting RNA to cDNA and then using the standard 16S bacterial primers and PCR conditions on it. Unfortunately all we get are giant streaks with no discernible...

03 December 2020 4,636 7 View

How do I make a cellulase solution using a powdered cellulase?

I have purchased a solid cellulase from Aspergillus niger (CAS Number 9012-54-8). I want to use it in solution as a positive control for CMC breakdown using agarose plate assays (incubate and use...

29 October 2019 4,798 2 View

What's the smallest sample of water from which once can get enough RNA for a metagenomic microbiome inventory?

I want to inventory the microbiome of small sources of water (say, the contents of a pitcher plant or a bromeliad, or even a puddle), using 16S rRNA. Most of the protocols and kits I found online...

27 June 2016 752 1 View

Has anyone had negative experiences with the 1KITE (1000 Insect Transcriptome Evolution) Project?

Low quality transcriptomes, contaminated data, publication restrictions, negative reviews on manuscript submissions that potentially compete with their work, suppression of other researchers......

01 January 1970 1,869 1 View