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Questions related from Masita Mandasari
Hello. I am working on a new project which profiling oral candidal species using ChromAgar from saliva. I am unfamiliar with this technique and the reference seems scarce and has limited info....
22 August 2017 9,472 1 View
Many papers working with CRISPR method present their indel mutations result by sequencing TA clones. If we can identify one mutation by direct sequencing of DNA sample, is it necessary to identify...
03 August 2015 218 7 View
I need to digest my plasmid using BamHI and SacI. Unfortunately, the enzymes came from different manufacturer, Fermentas and Wako Nippongene respectively. I checked the buffer ingredients and they...
25 July 2014 3,844 8 View
I'm new in southern blotting and I cannot troubleshoot my problems. I am trying to detect the transgene copy number in my transgenic mouse. I digest 3 ug of genomic DNA using EcoR1 (unique...
11 July 2014 3,544 2 View
My primer set was designed to yield 750 bp fragment. But, on the gel after PCR, I saw clear single band on 500 bp with very faint band around 750 bp. I purified the 500 bp fragment and did direct...
23 June 2014 1,082 11 View
