Maryam Zare

4 Questions 15 Answers 0 Followers

Questions related from Maryam Zare

Human T Cell activation using AntiCd3 AntiCd28

Hello I want to stimulate human T cells using AntiCD3 AntiCD28. The concentration of AntiCD3 antibody is 1mg/ml (eBioscience 16-0037-85). Based on protocols, I perform 1/1000 dilution to achieve...

05 May 2017 2,491 10 View

RNA isolation and cDNA synthesis from low number of cells(human regulatory T cells) ?

Hello I am working on Human regulatory T cells. I culture PBMCs and stimulate them. After that, I isolate subpopulation of regulatory T cells using MACS technique and then perform syber green...

10 October 2016 2,171 4 View

What is the appropriate wavelength for MTT assay?

Hello, I am performing MTT assay on PBMCs. For adjusting cell number, Briefly I seed different number of cells/well, incubate 24 and 48h, add MTT and incubate, finally add DMSO as solvent and read...

08 August 2016 1,449 14 View

What is the difference between Polyethylene Tetraphthalate and Polycarbonate membranes of Transwell Inserts?

Hello, I want to assess suppressive mechanisms (contact dependent& soluble Factors) of regulatory T cells using co-culture and Transwell experiments. Which one of the above membranes...

06 June 2016 9,242 2 View