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Questions related from Maryam Borhani Haghighi
I have samples of brain tissues which are over fixed due to prolonged exposure with paraformaldehyde. What can I do for getting appropriate signals in immunohistochemistry on these paraffin...
11 November 2016 4,503 9 View
I fixed the adherent cells with 4% paraformaldehyde for immunostaining after culturing cells on gelatin coated well of 48 well plate. After fixation I covered cells with enough amount of PBS...
10 October 2016 7,739 4 View
I would like to track cells after transplantation into the brain with PKH26 .I have several questions regarding to use of this dye: 1 .Does cellular stain with PKH26 last up to two months after...
05 May 2016 6,673 6 View
I want to study on mouses after birth, but unfortunately litters are eaten after birth by their mother.How can I prevent mothers from cannibalizing their litters?
04 April 2016 9,529 3 View
I need an animal model to induce perinatal specific area brain injury which reduce neurodegeneration by maternal injection of a substance during pregnancy that induced brain damage in embryos and...
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