Maryam Salaudeen

4 Questions 4 Answers 0 Followers

Questions related from Maryam Salaudeen

How to do a Mesenchymal stem cells migration assay in vitro?

Hello people, I plan to do a migration assay for some human bone marrow derived mesenchymal stem cells. According to the literature, these cells have a size ranging between 18 and 30um. What...

27 February 2025 3,552 2 View

How can I concentrate conditioned medium?

I plan to conduct an experiment in which conditioned medium of MSCs will be used as a therapeutic agent. Any idea how I can concentrate the medium before using it for treatment. Thanks.

23 April 2024 1,029 3 View

When is the best time to prime mesenchymal stem/stromal cells (MSCs)?

I want an optimal priming technique for my MSCs. I intend to use the conditioned medium from these primed cells to treat some inflamed cells. I am not sure whether to prime the cells as soon as...

18 January 2024 6,249 5 View

Is the hypoxia-inducing effect of CoCl2 reversible?

So I am trying to induce hypoxia in some mesenchymal stem cells using CoCl2. I am wondering if I need to change the media (containing CoCl2) after the induction/treatment time before assaying for...

05 September 2023 9,586 1 View