Martiniano M Ricardi

3 Questions 14 Answers 0 Followers

Questions related from Martiniano M Ricardi

Is Gibson assembly without Taq ligase possible?

Hi, I was recently told that Gibson assembly does not really need the use of Taq ligase. Once transformed, the bacteria can ligate the nicks on the vector with their endogenous enzymes. Has...

02 June 2021 3,685 3 View

I am wondering if once formed, the CRISPR ribonucleoprotein (crRNP) complex can loose the cr/tracrRNA and exchange it with another cr/tracrRNA?

Im new at CRISPR-CAS9 stuff and I am wondering if once formed, the CRISPR ribonucleoprotein (crRNP) complex can loose the cr/tracrRNA and exchange it with another cr/tracrRNA molecule. There is so...

24 April 2021 196 0 View

Can anyone share his/her experience purifying GFP (or GFP variants) in e-coli?

I will soon start expressing GFP-quimeric proteins in E. coli (BL21) and then purifying them using a 6xHIS tag. Does anybody have experience working with recombinant GFP proteins it complicated?...

14 January 2015 5,399 9 View